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目的:建立HPLC同时测定鼠掌老鹳草中没食子酸,原儿茶酸,槲皮素,山奈素,山奈素-3,7-O-α-二鼠李糖苷的含量测定方法。方法:采用RP-HPLC,Agilent TC-C18色谱柱(4.6 mm×250 mm,5μm),0.4%磷酸水溶液-甲醇为流动相梯度洗脱,流速0.8 mL·min-1,检测波长为切换波长,0～23 min,270 nm;23～55 min,370 nm,柱温30℃。结果:没食子酸,原儿茶酸,槲皮素,山奈素,山奈素-3,7-O-α-二鼠李糖苷的线性范围0.192～1.732 g·L-1(r=0.999 7),0.016～0.256 g·L-1(r=0.999 9),0.004～0.039 g·L-1(r=0.999 9),0.004～0.041 g·L-1(r=0.999 9),0.200 4～1.603 2 g·L-1(r=0.999 8),平均回收率(n=3)分别为99.93%(RSD 1.5%),98.27%(RSD 1.7%),100.07%(RSD 2.2%),99.92%(RSD 1.8%),99.63%(RSD1.0%)。结论:该含量测定的方法快速、准确、可靠、重复性好,可为药材的质量控制提供依据。
Objective: To establish a method for the simultaneous determination of gallic acid, protocatechuic acid, quercetin, kaempferol and kaempferol-3,7-O-α-rhamnoside in Geranium spp. METHODS: The mobile phase was eluted with RP-HPLC, Agilent TC-C18 column (4.6 mm × 250 mm, 5 μm) with a mobile phase of 0.4% phosphoric acid and methanol. The flow rate was 0.8 mL · min- 0 ~ 23 min, 270 nm; 23 ~ 55 min, 370 nm, column temperature 30 ℃. Results: The linear ranges of gallic acid, protocatechuic acid, quercetin, kaempferol and kaempferol-3,7-O-α-rhamnoside were 0.192-1.732 g · L -1 (r = 0.999 7) 0.016 ~ 0.256 g · L -1 (r = 0.999 9), 0.004 ~ 0.039 g · L -1 (r = 0.999 9), 0.004 ~ 0.041 g · L -1 (r = 0.999 9), 0.200 4 ~ 1.603 2 g · L-1 (r = 0.999 8), the average recoveries were 99.93% (RSD 1.5%), 98.27% 1.8%), 99.63% (RSD 1.0%). Conclusion: The determination of content of this method is rapid, accurate, reliable, reproducible, and can provide the basis for the quality control of medicinal materials.