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以ＢＵＦ７３１６Ａ肝癌细胞接种于ＢＵＦ大白鼠为荷瘤模型，同时施行单开腹、７０％肝切除及以上两种手术加术中输血。术后７～２０天测量肿瘤体积，并取单开腹及７０％肝切除术后５天的血清及脾粘着细胞，加入混合淋巴细胞培养。结果显示，７０％肝切除加术中输血与单开腹对比，肿瘤细胞生长速度明显增快（Ｐ＜０．０１，Ｐ＜０．０５）。在７０％肝切除加术中输血组，肿瘤体积最大。７０％肝切除术后的血清及脾粘着细胞，明显抑制Ｔ淋巴细胞的增殖（Ｐ＜０．０５）。此结果提示，肝部分切除和术中输血是临床上肝脏手术后残余肿瘤迅速生长的原因。肝部分切除后，机体可能产生免疫抑制因子，抑制免疫活性细胞的增殖，而术中输血加重这种免疫抑制状态，从而造成了有利于肿瘤细胞生长的机体环境。
The BUF rats were inoculated with BUF7316A hepatoma cells as a tumor-bearing model. At the same time, a single open abdomen, 70% hepatectomy, and more than two surgery plus blood transfusion were performed. Tumor volume was measured 7 to 20 days after surgery, and serum and spleen adherent cells were obtained from single open and 70% hepatectomy for 5 days and mixed lymphocyte culture was added. The results showed that 70% of liver resection plus intraoperative blood transfusion compared with single open abdomen, the tumor cell growth rate was significantly faster (P <0.01, P <0.05). In the 70% hepatectomy plus transfusion group, the tumor volume was the largest. After 70% hepatectomy, serum and spleen adherent cells significantly inhibited the proliferation of T lymphocytes (P<0.05). This result suggests that partial hepatectomy and intraoperative blood transfusion are clinically responsible for the rapid growth of residual tumors following liver surgery. After partial hepatectomy, the body may produce immunosuppressive factors that inhibit the proliferation of immunocompetent cells, and intraoperative transfusion exacerbates this immunosuppressive state, thereby creating an organismal environment conducive to tumor cell growth.