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取SD大鼠30只(200～300g)随机分为3组。20只动物用1.5％DCA-Na经胰管逆行注射诱发急性胰腺炎,其中半数于术前1小时,术后4小时以及细胞学实验前1小时经口给50％栀子煎剂各2ml,另一半给生理盐水,处理相同。术后24小时全部动物及10只未作处理的正常动物被处死,用肢原酶制备胰腺细胞悬液。在给CCK和氨甲酰胆硷前后测上清液中的淀粉酶检测细胞的反应性。实验揭示胰腺炎动物基础状态下淀粉酶的释放显著高于正常,但在CCK或氨甲酰胆硷刺激下的释放量则明显减少,然栀子组在刺激前后的释放量均显著地更少。结果表明基础状态下淀粉酶释放量的增多系炎症引起胰腺腺泡障害所致,而对刺激因子表现的反应性释放减少则可能是胰腺炎时的一种自身适应性保护机制,同时也表明栀子煎剂具有减轻这种障害和增强保护机制的作用。
30 SD rats (200-300 g) were randomly divided into 3 groups. Twenty animals were injected with 1.5% DCA-Na by retrograde injection of pancreatic duct to induce acute pancreatitis. Half of the animals were given 15% preoperatively, 4 hours postoperatively and 1 hour before cytology experiment. The other half gave saline and the same treatment. All animals and 10 untreated normal animals were sacrificed 24 hours after surgery and pancreatic cell suspensions were prepared using extremogenic enzymes. The reactivity of the cells was measured by measuring amylase in the supernatant before and after CCK and carbamylcholine. The experiment revealed that the amylase release was significantly higher than normal in the basal state of pancreatitis, but the release was significantly reduced under the stimulation of CCK or carbamylcholine, but the release amount was significantly lower before and after the stimulation. . The results showed that the increase of amylase release in the basal state was caused by inflammation and caused by acini of the pancreas. The decrease in the responsive release of stimulatory factors may be a self-adaptive protection mechanism of pancreatitis. The decoction has the effect of relieving this disorder and enhancing the protective mechanism.