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用具有内影像的nAchR配体抗体——银环蛇毒抗血清为工具,采用酶联免疫吸附法(ELISA)检测了81例重症肌无力(MG)患者血清中特异性相应的抗独特型抗体(Anti-Id),其中阳性38例,阳性率46.9％,用nAchR进行的ELISA特异性交叉抑制试验阳性。对各改良Osserman分型MG及MG是否伴胸腺瘤病人血清进行了Anti-Id ELISA检测,并与放射免疫沉淀法测定nAchR-Ab进行了比较,并对nAchR-配体抗体的Anti-Id在MG发病中的作用及Anti-Id的治疗前景作了探讨。
The specific anti-idiotypic antibodies in serum of 81 patients with myasthenia gravis (MG) were detected by enzyme-linked immunosorbent assay (ELISA) using the nAchR ligand antibody-bungarotoxin antiserum with internal images Anti-Id), of which 38 were positive, the positive rate of 46.9%, with nAchR ELISA-specific cross-inhibition test was positive. Anti-Id ELISA was performed on the sera of patients with modified Osserman type MG and MG with or without thymoma and compared with nAchR-Ab by radioimmunoprecipitation. Anti-Id of nAchR-Ligand antibody was measured in MG The role of pathogenesis and Anti-Id treatment prospects were discussed.