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目的和方法：为进一步阐明肿瘤血路转移的分子机制，采用活性染料玫瑰红摄入法，研究人卵巢癌细胞系ＨＯ－ ８９１０ 与激活的人脐静脉内皮细胞（ ＨＵＶＥＣｓ） 的粘附性以及抗粘附分子Ｅ－ 选择素（ Ｅ－ ｓｅｌｅｃｔｉｎ） 及其配体ｓＬｅｘ 单抗对ＨＯ－ ８９１０ 细胞与激活的ＨＵＶＥＣｓ 粘附的影响。结果：ＨＯ－ ８９１０ 细胞与血管内皮细胞的粘附能力明显高于低转性的人大肠癌ＨＲ－ ８３４８ 细胞。当血管内皮细胞被ＬＰＳ（１ μｇ／ｍ Ｌ） 激活后，ＨＯ－ ８９１０ 细胞与其的粘附较激活前增加３ 倍以上。用抗Ｅ－ ｓｅｌｅｃｔｉｎ 单抗处理血管内皮细胞或用其配体ｓＬｅｘ 的单抗处理肿瘤细胞，可分别将两细胞的粘附率阻断６２－５ ％ 和８１ ％ 。结论：Ｅ－ ｓｅｌｅｃｔｉｎ 和它的配体ｓＬｅｘ 是介导激活的血管内皮细胞与ＨＯ－ ８９１０ 细胞粘附反应的主要粘附分子
Objective and method: To further elucidate the molecular mechanism of tumor blood flow metastasis, the adhesion and anti-adhesion of human ovarian cancer cell line HO-8910 and activated human umbilical vein endothelial cells (HUVECs) were studied using the reactive dye rose red method. Effects of E-selectin (E-selectin) and Its Ligand sLex Monoclonal Antibody on Adhesion of HO-8910 Cells to Activated HUVECs RESULTS: The adhesion ability of HO-8910 cells to vascular endothelial cells was significantly higher than that of low-transformed human colon cancer HR-8348 cells. When vascular endothelial cells were activated by LPS (1 μg/m L), the adhesion of HO-8910 cells was more than 3 times higher than that before activation. Treatment of vascular endothelial cells with anti-E-selectin monoclonal antibodies or treatment of tumor cells with its ligand sLex monoclonal antibody blocked the adhesion rate of the two cells by 62-5 % and 81 %, respectively. CONCLUSION: E-selectin and its ligand sLex are major adhesion molecules mediating the adhesion of activated vascular endothelial cells to HO-8910 cells.