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目的探讨KiSS-1基因转染对骨肉瘤MG-63细胞株增殖的影响及其可能机制,为骨肉瘤的基因治疗提供实验室依据。方法将KiSS-1基因转染至骨肉瘤MG-63细胞株,应用免疫组化法检测MG-63-KiSS-1蛋白表达,CCK-8法检测细胞增殖情况,流式细胞术检测各期细胞比例。结果 MG-63-KiSS-1细胞KiSS-1蛋白高表达、增殖明显减慢、G2/M期细胞比例明显增高。结论 KiSS-1基因转染MG-63细胞后可抑制其增殖,其机制可能与阻滞细胞周期于G2/M期有关。
Objective To investigate the effect of KiSS-1 gene transfection on the proliferation of osteosarcoma MG-63 cell line and its possible mechanism to provide a laboratory basis for the gene therapy of osteosarcoma. Methods KiSS-1 gene was transfected into MG-63 osteosarcoma cell line. The expression of MG-63-KiSS-1 protein was detected by immunohistochemistry. The proliferation of MG-63 cells was detected by CCK-8 assay. Flow cytometry proportion. Results The expression of KiSS-1 protein in MG-63-KiSS-1 cells was significantly increased, the proliferation was significantly slowed down, and the proportion of cells in G2 / M phase was significantly increased. Conclusions KiSS-1 gene can inhibit the proliferation of MG-63 cells after transfection, which may be related to the arrest of cell cycle in G2 / M phase.