论文部分内容阅读
对玉米雄性不育系及保持系B73叶片超氧化物歧化酶(SOD)活性的测定表明,细胞溶质最高,玉米雄性不育系B73-C,B73-T及其保持系B73-N叶片基态超氧物歧化酶活性在细胞内的分布是:细胞溶质最高(占总活性77％);线粒体、叶绿体次之,且SOD活性N细胞质显著大于C和T细胞质;过氧化物酶(POX)的活性主要分布在线粒体和叶绿体中,占总活性85％左右。用专化于C细胞质的C毒素(HMC毒素)处理B73C、T与N三种细胞质玉米叶片后,这三种细胞溶质的SOD和POX酶活性均表现不敏感;三种细胞质的线粒体有不同反应:B73-C的SOD活性下降,导致细胞伤害,而B73-T和B73-N的SOD活性稍有提高或持平,保持了对C毒素的抗性。POX基本上随着SOD活性降低而增高或随着活性增高而降低。可见,HMC毒素能够诱导线粒体SOD和POX的活性,而这两个酶活性与玉米抗病性密切相关。
The determination of superoxide dismutase (SOD) activity in maize male sterile line B73-N leaves and its maintainer line B73 leaves showed that the highest cytosolic content was found in maize male sterile lines B73-C, B73-T and its maintainer B73-N leaves. Oxygen dismutase activity in intracellular distribution: cytosolic highest (accounting for 77% of the total activity); mitochondria, chloroplast second, and SOD activity of N cytoplasm was significantly greater than the C and T cytoplasm; peroxidase (POX) activity Mainly distributed in mitochondria and chloroplast, accounting for about 85% of total activity. The cytosolic SOD and POX activities of these three cytosols were both insensitive to treatment with C cytoplasmic C toxin (HMC toxin) on three cytoplasmic maize leaves, B73C, T and N; the mitochondria of the three cytoplasm had different responses : B73-C SOD activity decreased, leading to cell damage, while the B73-T and B73-N SOD activity slightly increased or flat, maintaining the resistance to C toxin. POX increases as SOD activity decreases or decreases as activity increases. It can be seen that HMC toxin can induce the activity of mitochondrial SOD and POX, and the activity of these two enzymes is closely related to the disease resistance of maize.