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建立了一种改良的血清1,25-双羟胆钙化醇(1,25-Dihydroxycholecalciferol,DHCC)超微量放射受体检测(RRA)技术。完成了灵敏度、精密度、准确度、稳定性及特异性等技术指标。报告了我国健康青年血清DHCC正常值;检测了先天性佝偻病、青春期佝偻病病人及患肾性骨病奶牛等血清DHCC水平。 根据配体与受体相互结合的定量关系,建立了DHCCR(DHCC受体)检测技术。在游离与结合配基分离方面,除建立与比较了DCC(葡聚糖包埋的活性炭)及HAP(羟基磷灰石)方法外,还首次将IEF(等电聚焦电泳)应用于DHCCR分离技术。对佝偻病鸡小肠粘膜上皮细胞受体含量进行了检测并比较了鸡小肠、输卵管壳腺及肝组织DHCCR含量。
A modified serum 1,25-dihydroxycholecalciferol (DHCC) ultra-trace radioactive receptor assay (RRA) was established. Completed the sensitivity, precision, accuracy, stability and specificity and other technical indicators. The normal serum DHCC values of healthy young people in China were reported. The levels of serum DHCC in patients with congenital rickets, adolescent rickets and cows suffering from renal osteodystrophy were detected. According to the quantitative relationship between ligand and receptor binding, DHCCR (DHCC receptor) detection technology was established. In addition to establishing and comparing DCC (dextran-embedded activated carbon) and HAP (hydroxyapatite) methods, IEF (isoelectric focusing electrophoresis) was applied to DHCCR separation technology . The contents of receptor of small intestinal mucosal epithelial cells in rickets were detected and the content of DHCCR in chicken intestine, fallopian tube gland and liver tissue was compared.