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为探讨低温冷藏对橡胶树胚性愈伤组织的影响,以(25±2)℃、常规增殖培养的胚性愈伤组织为对照,测定在(6±2)℃、添加外源抗氧化剂的低温保存培养基上生长25 d的胚性愈伤组织的相对生长量、抗氧化酶活性、恢复生长、体细胞胚诱导和植株再生能力.结果表明:低温冷藏胚性愈伤组织的相对生长量比对照的显著降低83.1％,但细胞活力相对强;低温冷藏胚性愈伤组织的SOD、POD、CAT和APX等抗氧化酶活性与对照无显著差异,但GR酶活性比对照的显著增加4.21倍;恢复(25±2)℃、正常增殖培养,胚性愈伤组织的存活率、相对生长量、体细胞胚诱导率与对照的无显著差异,但植株再生能力提高47.2％.综上所述,低温冷藏条件下,外源抗氧化剂能有效保护橡胶树胚性愈伤组织,可进一步优化保存条件以延长保存时间,为今后利用低温保存橡胶树优质胚性愈伤组织,避免因频繁继代引起胚性愈伤组织生长和胚性的衰退,妥善保存橡胶树种质资源提供理论依据和技术保障.“,”The rubber tree (Hevea brasiliensis) is an important economic crop which has a large-scale cultivation in the tropical and subtropical regions. In order to investigate response of embryogenic calli of Hevea brasiliensis to low-temperature storage with exogenous antioxidants, using calli growing on conventional proliferation medium under (25±2)℃ as the control, we measured the relative growth, antioxidant enzyme activities, recovery growth, somatic embryo induction and plant regeneration ability of the calli cultivated for 25 d under (6±2)℃. The results indicated that, compared with the control, the relative growth of the embryogenic calli growing at low temperature decreased markedly by 83.1％, but the calli cell viabilities were stronger than those of the control calli. Compared with the control, the activities of antioxidases, such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) in embryogenic calli were not significantly different from those of the control calli, but the activity of glutathione reductase (GR) increased significantly by 4.21 times. After recovering growing under (25±2)℃, compared with those of the control, there were no significant differences in the calli survival rate, relative growth, and somatic embryogenesis rate of embryogenic calli cultivated at low temperature, but the plant regeneration capacity increased by 47.2％. In summary, under low temperature refrigeration condition, exogenous antioxidants could protect rubber embryogenic calli effectively. The cell activities of embryogenic calli in Hevea brasiliensis were not destroyed significantly under (6±2)℃for 25 d. We can further optimize the conserving conditions to extend the preservation time to obtain an ideal conserving method. Then, it can provide theoretical basis and technical support for using low temperature conditions to have a short to medium term storage for embryogenic calli to avoid cell embryonic and growth decline due to frequent subculture, and have a proper preservation of rubber tree germplasm resources.