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目的:探讨益气温阳活血方对慢性心力衰竭心室重塑大鼠转录因子GATA结合蛋白4(GATA4)的影响。方法:通过异丙肾上腺素诱导慢性心力衰竭动物模型。健康Wistar大鼠40只,随机分为4组:正常组、模型组、西药组(卡维地洛治疗组)、中药组(益气温阳活血方治疗组),每组10只;计算左、右心室质量指数,应用ELISA法检测心肌组织Ⅰ型和Ⅲ型胶原含量,计算Ⅰ型/Ⅲ型胶原比值,以RT-PCR法以及Western Blot法检测GATA4 mRNA及蛋白质表达水平。结果:模型组左心室质量指数(LVMI)、Ⅰ型胶原含量、Ⅰ型/Ⅲ型胶原比值及GATA4 mRNA与蛋白质的表达水平较正常组明显升高(P<0.05);中药组能够明显减少Ⅰ型胶原含量,降低Ⅰ型/Ⅲ型胶原比值,抑制GATA4 mRNA与蛋白质的表达水平,与模型组比较差异有统计学意义(P<0.05),而与西药组比较差异无统计学意义。结论:益气温阳活血方通过抑制GATA4的表达减少Ⅰ型胶原含量,降低Ⅰ型/Ⅲ型胶原比值,逆转心室重塑,改善心脏功能。
Objective: To investigate the effect of Yiqi Wenyang Huoxue Recipe on GATA4 (GATA4) in ventricular remodeling rats with chronic heart failure. Methods: An animal model of chronic heart failure was induced by isoproterenol. Forty healthy Wistar rats were randomly divided into 4 groups: normal group, model group, western medicine group (carvedilol treatment group), Chinese medicine group (Yiqi Wenyang Huoxue Recipe treatment group) The right ventricular mass index, myocardial collagen type Ⅰ and type Ⅲ were measured by ELISA. The ratio of type Ⅰ / type Ⅲ collagen was calculated. The expression of GATA4 mRNA and protein was detected by RT-PCR and Western Blot. Results: The levels of LVMI, type Ⅰ collagen, type Ⅰ / Ⅲ collagen and GATA4 mRNA and protein in model group were significantly higher than those in normal group (P <0.05) (P <0.05), but there was no significant difference compared with the western medicine group (P> 0.05) .Conclusion: The expression of GATA4 mRNA and protein in the model group is lower than that in the model group. Conclusion: Yiqi Wenyang Huoxue Recipe can reduce type Ⅰ collagen content, decrease type Ⅰ / Ⅲ collagen ratio, reverse ventricular remodeling and improve cardiac function by inhibiting the expression of GATA4.