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群体的构建与遗传结构分析是利用该群体开展进一步研究的基础。选用157对均匀分布在水稻12条染色体上且在双亲间具有多态性的微卫星标记分别对“培矮64S/Nipponbare”F_2群体(180个株系)和F6群体(330个株系)进行分析,分别对单个微卫星位点的群体分布、株系的遗传成分以及纯合进度进行分析。结果表明:各遗传位点和各株系的遗传成分表现连续分布,一些位点呈显著或极显著偏分离,双亲对株系呈不同的遗传贡献率,某些株系对亲本具有偏爱性;各位点和株系的纯合进度呈连续分布,分别与F_2、F_6杂异位点呈极显著正、负相关。这将有助于我们充分的认识籼粳交后代群体遗传结构特征,并为群体结构优化提供依据。
The construction of population and the analysis of genetic structure are the bases for further research using this group. A total of 157 pairs of “Pei’ai 64S / Nipponbare” F2 population (180 lines) and F6 population (330 lines) were selected from 157 pairs of microsatellite markers evenly distributed on 12 chromosomes of rice and their parents were polymorphic. ) Were analyzed, respectively, the population distribution of single microsatellite loci, genetic components of lines and homozygous progress were analyzed. The results showed that the genetic components of each locus and each strain showed a continuous distribution with some sites showing significant or very significant segregation. The parents showed different genetic contribution rates to the strains, and some strains had a preference for the parents. The homozygous progress of each locus and strain showed a continuous distribution, which showed extremely significant positive and negative correlation with F_2 and F_6 loci respectively. This will help us to fully understand the genetic structure of progenies of indica and japonica offspring, and provide the basis for population structure optimization.