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背景:YM14673为促甲状腺激素释放激素(thyrotropin-releasinghormone,TRH)类似物,具有脑保护作用。目前的许多研究都在探讨其脑保护作用是否通过脑损伤后对脑水肿形成的抑制而实现的。目的:观察TRH类似物YM14673对脑损伤后血脑屏障的影响。设计:随机对照的实验研究。地点和对象:实验地点:北京市神经外科研究所;研究对象为Wistar大鼠60只,随机分为假手术组、生理盐水组、给药Ⅰ组(0.1mg/kg)、给药Ⅱ组(1mg/kg),每组15只动物。干预:制作大鼠急性脑损伤模型,创伤前从股静脉注入伊文氏蓝用于测定血脑屏障通透性的改变。假手术组开骨窗,不进行创伤打击。其他组行创伤打击。打击后腹腔注射生理盐水或YM14673溶液,伤后24h处死,测定脑水含量及脑组织和血浆中的伊文氏蓝含量。主要观察指标:①各组大鼠左右半球脑水含量变化。②各组大鼠左右半球及血浆中伊文氏蓝含量变化。结果:大鼠脑损伤后,脑组织脑水含量生理盐水组明显高于假手术组犤左侧半球:生理盐水组为(79.57±0.47)%,假手术组为(78.29±0.63)%,t=3.98,P<0.01犦,遭受直接打击的左侧半球其脑水含量明显高于右半球(t=4.74,P<0.01)。给药物YM14673干预后左右半球的脑水含量均显著下降犤左侧半球:给药Ⅰ组(0.1mg/kg)为(78.95±0.21)%,给药Ⅱ组(1mg/kg)为(78.92
BACKGROUND: YM14673 is a thyrotropin-releasing hormone (TRH) analogue with a neuroprotective effect. Many current studies are investigating whether their brain protective effect is achieved through the inhibition of brain edema formation after brain injury. Objective: To observe the effect of TRH analog YM14673 on blood-brain barrier after brain injury. Design: Randomized controlled experimental study. Sixty Wistar rats were randomly divided into sham operation group, saline group, administration group Ⅰ (0.1 mg / kg), administration group Ⅱ (control group) 1 mg / kg), 15 animals per group. Intervention: A rat model of acute brain injury was made. Evans blue was injected from the femoral vein before trauma to determine changes in the permeability of the blood-brain barrier. Sham operation group osteoporosis, no trauma attack. Other group trauma hit. After intraperitoneal injection of physiological saline or YM14673 solution, the mice were sacrificed 24 hours after injury, and the content of brain water and the content of Evans blue in brain tissues and plasma were measured. MAIN OUTCOME MEASURES: ① Changes of cerebral water content in left and right hemispheres of rats in each group. ② The contents of Evans blue in the left and right hemispheres and plasma of rats in each group. Results: After brain injury in rats, brain water content in brain tissue was significantly higher in the saline group than in the sham operation group (79.57 ± 0.47%) in the saline group and (78.29 ± 0.63)% in the sham group = 3.98, P <0.01 犦. The left hemisphere subjected to direct attack had a significantly higher brain water content than the right hemisphere (t = 4.74, P <0.01). After administration of YM14673, the brain water content in the left and right hemispheres decreased significantly (P <0.05). The left hemisphere of the left hemisphere was significantly reduced (78.95 ± 0.21)% in the group Ⅰ and (78.92 ± 0.21)%,