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以莱克多巴胺为模板分子,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,乙醇为致孔剂采用沉淀聚合法合成了对莱克多巴胺具有高特异识别性的分子印迹聚合物,平衡吸附量达145.6μg/g微球。将制备的印迹聚合物微球装填高效液相色谱柱(50 mm×4.6 mm i.d.)。印迹柱的印迹因子(IF)为1.2,印迹柱和非印迹柱的保留因子(k)分别为33.3和15.1,表明合成聚合物印迹效果明显。分别用10μg/mL克伦特罗、沙丁胺醇和莱克多巴胺3种β-兴奋剂溶液考察自制高效液相色谱柱的分离效果,克伦特罗和沙丁胺醇的选择性系数(α)分别为2.3和1.2,表明所制备的分子印迹柱对以上3种β-兴奋剂显示了良好的选择性。
Using ractopamine as a template molecule, methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linking agent, and ethanol as a porogen, a molecularly imprinted molecularly imprinted polymer with high specificity for ractopamine was synthesized by precipitation polymerization Polymer, the equilibrium adsorption capacity of 145.6μg / g microspheres. The prepared imprinted polymer microspheres were packed with high performance liquid chromatography (50 mm × 4.6 mm i.d.). The imprinting factor (IF) of the imprinting column was 1.2, and the retention factors (k) of the imprinting and non-imprinting columns were 33.3 and 15.1, respectively, indicating that the imprinting effect of the synthetic polymer was significant. The separation efficiency of home-made high performance liquid chromatography (HPLC) columns was investigated using 10 μg / mL clenbuterol, salbutamol and ractopamine respectively. The selectivity coefficients of clenbuterol and salbutamol were 2.3 and 1.2, respectively , Indicating that the prepared molecularly imprinted column showed good selectivity for the above three β-agonists.