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目的建立同时测定槐角丸中槐角苷、黄芩苷、染料木素和汉黄芩素4种有效成分含量的HPLC方法。方法采用Dia-monsil C18柱(4.6 mm×200 mm,5μm)进行分离;流动相为乙腈-0.05%磷酸溶液,梯度洗脱(0~10 min,25%~37%乙腈;10~25 min,37%~60%乙腈);检测波长260 nm;柱温30℃;流速1.0 mL.min-1;进样量10μL。结果槐角苷、黄芩苷、染料木素和汉黄芩素的质量浓度与峰面积分别在11.6~116μg.mL-1(r=0.999 6,n=6)、33.5~335μg.mL-1(r=0.999 8,n=6)、0.46~4.63μg.mL-1(r=0.999 8,n=6)和1.54~15.4μg.mL-1(r=0.999 7,n=6)内呈良好的线性关系;平均加样回收率依次为99.3%、99.6%、99.6%和99.0%。RSD为1.1%、1.1%、1.4%和0.8%。结论该方法快速、简便、重复性好,适合于同时测定槐角丸中槐角苷、黄芩苷、染料木素和汉黄芩素的含量。
OBJECTIVE To establish an HPLC method for the simultaneous determination of 4 kinds of active ingredients in Huaiji pills, including baicalin, baicalin, genistein and wogonin. Methods The separation was performed on a Dia-monsil C18 column (4.6 mm × 200 mm, 5 μm). The mobile phase consisted of acetonitrile-0.05% phosphoric acid solution with gradient elution (0-10 min, 25% -37% 37% -60% acetonitrile); detection wavelength was 260 nm; column temperature was 30 ℃; flow rate was 1.0 mL.min-1; injection volume was 10 μL. Results The mass concentration and peak area of acacia glycosides, baicalin, genistein and wogonin were 11.6-116 μg.mL-1 (r = 0.999 6, n = 6) and 33.5-335 μg.mL-1 (R = 0.999 8, n = 6), 0.46 to 4.63 μg.mL-1 (r = 0.999 8, n = 6) and 1.54 to 15.4 μg.mL-1 The average recovery was 99.3%, 99.6%, 99.6% and 99.0%, respectively. RSD was 1.1%, 1.1%, 1.4% and 0.8%. Conclusion The method is rapid, simple and reproducible. It is suitable for the simultaneous determination of acarbose, baicalin, genistein and wogonin in Huaiji pills.