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乙型肝炎病毒(HBV)是一种独特的嗜肝病毒,其脱氧核糖核酸(DNA)在受染者体内的存在状态是彼此相异但又密切相关的,对这些状态的研究使我们能不断加深对乙肝发病机理的认识。本文就近几年HBV-DNA在人体内存在状态的研究进展作一简要综述。一、血清内HBV-DNA的状态及其意义许多学者均证实应用斑点杂交法检测血清HBV-DNA是一种特异、敏感、简便的方法。Feinman的实验结果表明,用放射免疫法检测HBsAg的血清稀释度为10~(-5),而用斑点杂交法检测HBV-DNA的血清稀释度可达10~(-8)以上,许多HBsAg、HBe-
Hepatitis B virus (HBV) is a unique hepadnavirus whose DNA is distinct but closely related to each other in the affected person’s body. Research into these conditions has allowed us to continue Deepen the understanding of the pathogenesis of hepatitis B. In this paper, in recent years, the status of HBV-DNA in the human body to make a brief review of research progress. First, the serum HBV-DNA status and its significance Many scholars have confirmed the use of dot blot hybridization detection of serum HBV-DNA is a specific, sensitive and easy way. Feinman’s experimental results showed that the serum dilution of HBsAg was 10 ~ (-5) by radioimmunoassay, while the serum dilution of HBV-DNA by dot blot hybridization was more than 10 ~ (-8). Many HBsAg, HBe-