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长期以来,免疫学家们梦想根据选择的特定抗原,生产大量纯抗体,这种特定抗原可能存在于致病病毒或癌细胞的表面。由于杂交瘤(Hybridoma)技术的成功,使这一梦想变为现实。杂交瘤是由一个骨髓瘤细胞与一个激活的B细胞融合在一起形成的,融合的大细胞忠实地保留了两个原细胞不断增殖并积极生产抗体的特征。由于每个杂交瘤来源于同一种B细胞,当单个杂交瘤细胞生长为一团细胞即杂交瘤细胞“克隆”时,其所产生的同一种抗体即
For a long time, immunologists have dreamed of producing large amounts of pure antibodies based on the specific antigen of choice, and this particular antigen may be present on the surface of pathogenic viruses or cancer cells. Due to the success of Hybridoma technology, this dream has come true. Hybridomas are formed by the fusion of one myeloma cell with one activated B cell. The fused macrophages faithfully retain the features of both proliferating and actively producing antibodies. Since each hybridoma is derived from the same B cell, when a single hybrid cell grows into a cluster of cells, that is, a “clone” of a hybridoma, the same antibody it produces