豚鼠耳蜗结构与人类相似,广泛用于听力学实验及基础研究。其耳蜗扫描电镜样品的制备较复杂繁琐,在制备过程中易产生收缩及龟裂等人工假象,且耗用大量昂贵的锇酸。我们在参考国内外文献方法基础上进行了某些摸索改进,制备的样品达到了满意的结果,现介绍如下。 Guinea pig cochlear structure similar to humans, widely used in experimental and basic audiology. The preparation of cochlear SEM samples is complicated and complicated. Artificial artifacts such as shrinkage and cracking are easy to produce in the preparation process, and consume a large amount of expensive osmium tetroxide. We have made some groping improvements based on the references of domestic and foreign literature methods, and the prepared samples have achieved satisfactory results and are presented as follows.