论文部分内容阅读
将转有报告基因ｌａｃＺ的成纤维细胞３Ｔ３／ＢＡＧ以５×１０５量尾静脉注射到Ｂａｌｂ／ｃ小鼠体内，７ｄ后在主要脏器检测到３Ｔ３／ＢＡＧ的表达旦至少可持续３０ｄ．将转有人凝血因子Ⅸ小基因（ｈＦⅨｍｉｎｉｇｅｎｅ）的正向表达载体Ｇ１ＮａＣｉ＇和反向表达载体Ｇ１ＮａＣｉ＇ⅨＲ的成纤维细胞ＰＡ３１７／Ｇ１ＮａＣｉ＇Ⅸ和ＰＡ３１７／ＧＩＮａＣｉ＇ⅨＲ分别以１×１０＇量尾静脉注射到Ｂａｌｂ／ｃ小鼠体内，ＥＬＩＳＡ测定小鼠血浆中ｈＦⅨ蛋白的表达量，１６ｄ后，注射ＰＡ３１７／Ｇ１ＮａＣｉ＇Ⅸ的小鼠表达为（８．８９±０．５８）ｎｇ／ｍｌ，注射ＰＡ３１７／ＧＩＮａＣｉ＇ⅨＲ的小鼠的表达为（６９７±１９８）ｎｇ／ｍｌ．而只注射ＰＡ３１７细胞的小鼠表达一直维持在基底水平．
The 3T3 / BAG fibroblasts transfected with reporter gene lacZ were injected into Balb / c mice via tail vein at a dose of 5 × 105. After 3 days, the expression of 3T3 / BAG was detected in major organs for at least 30 days. The fibroblasts PA317 / G1NaCi’IX and PA317 / GINaCi’IXR, which were transfected with the positive expression vector G1NaCi ’of hFⅨminigene and the reverse expression vector G1NaCi’IXR, were respectively treated with 1 × 10’ tail vein The expression of hFⅨ protein in the plasma of mice was measured by ELISA. After 16 days, the mice injected with PA317 / G1NaCi’Ⅸ were expressed as (8.89 ± 0.58) ng / ml, injected with PA317 / GINaCi’IXR mice was (697 ± 198) ng / ml. However, mice injected with PA317 alone maintained their basal expression.