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在不同温度和不同光照条件下对 3株金龟子绿僵菌和 1株贵州绿僵菌的液体深层培养研究表明 ,光照对菌丝生长和液生分生孢子产量均无显著影响 .但培养温度显著影响菌丝生长和液生分生孢子的产出率 ,2 5～ 2 8℃为液体深层培养绿僵菌的适宜温度 ,其中以 2 8℃最佳 .对 3～ 4龄马尾松毛虫幼虫的室内毒力测定表明 ,绿僵菌液生分生孢子的毒力略低于气生分生孢子 .表 5参 15
The deep culture of three Metarhizium anisopliae and one Metarhizium anisopliae under different temperatures and light conditions showed that there was no significant effect of light on mycelial growth and conidial production but the temperature was significant The mycelial growth and the rate of liquid conidiospore production were affected, and the suitable temperature for the culture of Metarhizium anisopliae was 25 ~ 28 ℃, and the optimum temperature was 28 ℃ .For 3 ~ 4 instar larvae of Dendrolimus punctatus Toxicity determination of indoor showed that the virulence of Metarhizium anisopliae conidia was slightly lower than that of aerial conidia.