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将硫堇电聚合在光透电极的表面,再利用壳聚糖将黄嘌呤氧化酶固定在具有光电活性的聚硫堇光透电极的表面上制备了光致电化学鸟嘌呤传感器.基于同时具有光敏和电子受体功能的聚硫堇光电界面,该传感器能与黄嘌呤氧化酶催化鸟嘌呤氧化而产生的电子供体(过氧化氢)产生光致电化学响应,通过测量光致电化学反应产生的光电流实现了对鸟嘌呤的检测.文中探讨了传感器的光致电化学响应机理,讨论了偏压、酶量、电解质溶液pH对传感器测定鸟嘌呤的影响.在优化的实验条件下,该传感器对鸟嘌呤的测定范围为1.00～200μmol/L,检出限为0.55μmol/L,9次测定的相对标准偏差小于3.92%.应用该传感器对酸解DNA脱出的鸟嘌呤基和药品阿昔洛韦进行的检测实验显示,相对标准偏差小于5.37%,加标回收率为96.8%～106%.该传感器的制备和对鸟嘌呤的检测不需要过氧化物酶,不需要除氧,有经济、简便等优点.
The thionine was electropolymerized on the surface of the light-transmitting electrode, and the xanthine oxidase was immobilized on the surface of the photo-active polythionine transparent electrode by using chitosan to prepare a photoelectrochemical guanine sensor. And electron acceptor function of the polythionine photoelectric interface, the sensor can xanthine oxidase catalyzed guanine oxidation resulting electron donor (hydrogen peroxide) to produce a photoelectrochemical response, by measuring the light generated by the electrochemical reaction of light The detection of guanine is realized by the current.In this paper, the mechanism of the photoelectrochemical response of the sensor is discussed and the influence of the bias voltage, the amount of the enzyme and the pH of the electrolyte solution on the determination of guanine by the sensor are discussed. Under optimized experimental conditions, The detection limit of purine is 1.00 ~ 200μmol / L, the detection limit is 0.55μmol / L, the relative standard deviation of 9 determinations is less than 3.92% .Using this sensor, the guanosine base and drug acyclovir, The detection experiment showed that the relative standard deviation was less than 5.37% and the recovery was 96.8% ~ 106% .The preparation of the sensor and the detection of guanine did not require peroxidase, , Economical, simple and so on.