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以生物素（ｂｉｏｔｉｎ１６ｄＵＴＰ）标记的天蓝冰草（Ａｇｒｏｐｙｒｏｎｉｎｔｅｒｍｅｄｉｕｍ（Ｈｏｓｔ）Ｐ．Ｂ．＝Ｅｌｙｔｒｉｇｉａｉｎｔｅｒｍｅｄｉａ（Ｈｏｓｔ）Ｎｅｖｓｋｉ＝Ｔｈｉｎｏｐｙｒｕｍｉｎｔｅｒｍｅｄｉｕｍ（Ｈｏｓｔ）ＢａｒｋｗｏｒｔｈａｎｄＤｅｗｅｙ）染色体组ＤＮＡ为探针，普通小麦（ＴｒｉｔｉｃｕｍａｅｓｔｉｖｕｍＬ．）“中国春”ＤＮＡ为封闭，用荧光原位杂交（ＦＩＳＨ）技术对小冰麦３３号进行检测。结果表明：在一对染色体的端部显现出绿色荧光，这说明小冰麦３３号携带外源基因的冰草染色体片段位于小麦染色体端部，而且易位的染色体片段是较小的。从ＤＮＡ水平直接证明小冰麦３３是一个冰草染色体片段易位到小麦染色体端部的易位系。
Using biotin-16-dUTP-labeled genomic DNA as a probe, Triticum aestivum (Host) P.B. = Elytrigia intermedia (Host) Nevski = Thinopyrum intermedium (Host) BarkworthandDewey) The “Chinese Spring” DNA was closed and the Xiaobingmai 33 was detected by fluorescence in situ hybridization (FISH). The results showed that green fluorescence appeared at the end of a pair of chromosomes, which indicated that the fragment of Agropyron chinensis carrying the exogenous gene was located at the end of wheat chromosome, and the translocated chromosome fragment was smaller. Direct evidence from the DNA level that the barley 33 is a translocation of a barley grass chromosome fragment to the end of wheat chromosomes.