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目的探讨δ-生育三烯酚对人结肠癌细胞SW620细胞增殖抑制作用,研究Notch信号通路中关键蛋白在δ-生育三烯酚抑制结肠癌细胞增殖中的表达及意义。方法以终浓度分别为5、10、15、20、25、30μmol/L的δ-生育三烯酚培养SW620细胞,应用噻唑蓝(MTT)试验观察δ-生育三烯酚抑制结肠癌细胞增殖的作用,计算细胞存活率。应用免疫细胞化学方法检测Notch信号通路关键蛋白Notch、Jagged的表达变化。观察浓度为1μg/ml放线菌酮(CHX)对生育三烯酚抑制结肠癌细胞增殖作用的影响。结果随着δ-生育三烯酚浓度的增加,SW620细胞的存活率逐渐降低,差异有统计学意义(P<0.05)。Notch信号通路关键蛋白Notch1、Jagged1在溶剂对照组结肠癌细胞中表达量最高,随着加入生育三烯酚剂量的增加,表达量逐渐降低。经CHX干预过的细胞,再加入δ-生育三烯酚,作用24 h后,细胞存活率(83.33%)较单独加入生育三烯酚作用的细胞存活率(28.51%)显著增高,差异有统计学意义(P<0.05)。结论生育三烯酚抑制人结肠癌细胞增殖并诱导其死亡的方式可能为paraptosis样死亡,Notch信号通路中关键蛋白参与了这一过程。
Objective To investigate the inhibitory effect of δ-tocotrienol on the proliferation of human colon carcinoma cell line SW620 and to study the expression and significance of key proteins in Notch signaling pathway in the inhibition of proliferation of colon cancer cells by δ-tocotrienol. Methods SW620 cells were cultured with δ-tocotrienol at the final concentrations of 5, 10, 15, 20, 25 and 30 μmol / L, respectively. MTT assay was used to observe the inhibitory effect of δ-tocotrienol on the proliferation of colon cancer cells Effect, calculate cell viability. Immunocytochemistry was used to detect Notch and Jagged expression changes of Notch signaling pathway. The effect of cycloheximide at a concentration of 1μg / ml on the proliferation of colon cancer cells was observed. Results As the concentration of δ-tocotrienol increased, the survival rate of SW620 cells gradually decreased, with statistical significance (P <0.05). Notch signaling pathway key proteins Notch1, Jagged1 in the solvent control group colon cancer cells in the highest expression, with the addition of tocotrienol dose increased, the expression decreased. After 24 h treatment, cell viability (83.33%) was significantly higher than that of cells treated with CHX alone (28.51%) with the addition of δ-tocotrienol Significance (P <0.05). Conclusions Tocotrienol inhibits the proliferation and induces the death of human colon cancer cells in a paraptosis-like manner. Key proteins in the Notch signaling pathway are involved in this process.