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目的:研究颗粒溶素肽对白念珠菌的毒性作用。方法:将白念珠菌和不同浓度的颗粒溶素肽混合培养后,计算沙氏琼脂培养基平板上白念珠菌菌落数。采用标准微量稀释法测定颗粒溶素肽以及氟康唑(FCZ)对白念珠菌的最低抑菌浓度(MIC)。结果:当相应于颗粒溶素第2和第3个螺旋的肽段G2和G3浓度达到20μg/ml时,对白念珠菌产生较强的细胞毒性,形成的平均菌落数分别从883、937降到203、218,而G1、G4、G5肽段浓度达到40μg/ml时,也不能明显减少白念珠菌平均菌落数。3株标准白念珠菌株对颗粒溶素肽均较为敏感,MIC分别为26、22、29μg/ml,而对FCZ的MIC分别为4、20、128μg/ml。结论:颗粒溶素肽对白念珠菌具有细胞毒性,是人体内天然存在的抗真菌感染物质之一,具有潜在的药物开发意义。
Objective: To study the toxic effect of granulysin on Candida albicans. Methods: Candida albicans and different concentrations of granulysin peptide were mixed culture, the number of Candida albicans colonies were calculated on Sabouraud agar medium. Minimal inhibitory concentrations (MICs) of granulysin and fluconazole (FCZ) against C. albicans were determined by standard microdilution methods. Results: Candida albicans produced strong cytotoxicity when the concentrations of G2 and G3 corresponding to the second and third helices of granulysin reached 20μg / ml. The average number of colonies formed decreased from 883,937 203,218, while the G1, G4, G5 peptide concentration reached 40μg / ml, they can not significantly reduce the average number of Candida albicans. Three strains of Candida albicans were sensitive to granulysin, with MICs of 26, 22, and 29 μg / ml, respectively, while MICs of FCZ were 4, 20 and 128 μg / ml, respectively. CONCLUSION: Granulysin is cytotoxic to Candida albicans and is one of the naturally occurring antifungal agents in human body. It has the potential of drug development.