论文部分内容阅读
目的:探讨人参属4种药材人参、西洋参、三七、竹节参的鉴别方法及其系统发育关系。方法:扩增线粒体nad1基因,测序比对,分析该基因序列的差异。结果:获得人参(移山参、高丽参)和竹节参该基因的1290 bp片段序列,西洋参和三七的该基因片段长度则分别为1269 bp和1522 bp,主要差异为该基因的b/c内含子。在NJ系统发育树上,人参和竹节参关系最近,其次是西洋参,而与三七的关系最远。结论:基于该片段的碱基差异可从这4种药材中将西洋参、三七鉴别出来。人参属植物线粒体nad1基因b/c内含子存在一定的进化信息,可以进一步用于人参属药材的鉴别及系统发育关系分析。
Objective: To explore the identification method and phylogenetic relationship of four kinds of medicinal materials, Panax ginseng, Panax quinquefolium, Panax notoginseng and Panax japonicus. Methods: The mitochondrial nad1 gene was amplified and sequenced. The sequence differences of the gene were analyzed. Results: The 1290 bp fragment of this gene was obtained from Panax ginseng (Panax ginseng, Ginseng) and Panax japonicus. The length of this gene was 1269 bp and 1522 bp, respectively. The main difference was the b / c intron. In NJ phylogenetic tree, the relationship between ginseng and bamboo is the closest, followed by American ginseng, but the relationship with the most distant. Conclusion: Based on the base difference of this fragment, American ginseng and Panax notoginseng can be identified from these four medicinal materials. There is some evolutionary information about the b / c intron of the ginseng plant mitochondrial nad1 gene, which can be further used for the identification and phylogenetic analysis of Panax species.