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目的 研究 Sj C2 3蛋白质疫苗对 Sj C2 3DNA疫苗增强小鼠的免疫保护作用的效果。方法 分别构建和制备 Sj C2 3DNA疫苗 (pc DNA3.1- Sj C2 3)和 Sj C2 3大亲水片段融合蛋白质 (GST-HD)疫苗。把 48只 BAL B/ c小鼠随机分为 A、B、C、D 4组 ,每组 12只。 A组 (DNA和蛋白质疫苗联合免疫组 )每只小鼠分别在第 0、2周经股四头肌注射 10 0μg Sj C2 3质粒 DNA,在第 4周经背部皮下多点注射 5 0 μg GST- HD+5 0 μg CFA;B组 (Sj C2 3DNA组 )每只小鼠分别在第 0、2、4周经股四头肌注射 10 0 μg Sj C2 3质粒 DNA;C组 (蛋白质疫苗组 )于第 4周每鼠经背部皮下多点注射 5 0 μgGST- HD+5 0 μg CFA1次 ;D组 (对照组 )每鼠分别在第 0、2、4周肌注 10 0 μg pc DNA3.1。各组在末次免疫后 4周每鼠以 45± 2条日本血吸虫尾蚴攻击感染 ,攻击后 45 d剖杀小鼠 ,计数成虫及肝内虫卵数。在免疫前、末次免疫后和攻击后从小鼠尾静脉采血 ,分离血清 ,用 Western- blot法检测抗体反应。结果 联合组与质粒对照组相比 ,获得了 42 .9%的减虫率和 6 0 .0 %的减卵率 ,均显著高于单独Sj C2 3DNA疫苗组 2 8.1%的减虫率和 37.9%的减卵率 ,及单独蛋白质疫苗组 (GST- HD) 2 3.8%减虫率和 39.5 %的减卵率 (P均 <0 .0 5 )。联合组抗体水平?
Objective To investigate the effect of Sj C2 3 protein vaccine on immune protection in mice induced by Sj C2 3DNA vaccine. Methods Sj C2 3DNA vaccine (pc DNA3.1-Sj C2 3) and Sj C2 3 large hydrophilic fragment fusion protein (GST-HD) vaccine were constructed and prepared respectively. 48 BAL B / c mice were randomly divided into A, B, C, D 4 groups, 12 in each group. In group A (DNA and protein vaccine combined immunization), mice were injected with 0 0 μg Sj C2 3 plasmid DNA respectively through the quadriceps muscle at week 0 and week 2, and subcutaneously injected with 50 μg GST - HD + 50 μg CFA; group B (Sj C2 3DNA group), mice were injected with 10 0 μg Sj C2 3 plasmid DNA at the 0th, 2nd, 4th and 4th week respectively. Group C (protein vaccine group ) Were injected subcutaneously with 50 μg of GST-HD and 50 μg of CFA once a day for the fourth week. Rats in group D (control group) were intramuscularly injected with 100 μg pcDNA3 at weeks 0, 2 and 4, respectively. 1. At 4 weeks after the last immunization, each group was challenged with 45 ± 2 S. cercariae cercariae, and the mice were sacrificed 45 d after challenge. The numbers of adult and intrahepatic egg were counted. Blood was collected from the tail vein of mice before and after the last immunization, and after the challenge, the serum was separated and the antibody reaction was detected by Western-blot. Results Compared with the plasmid control group, the worm reduction rate of 42.9% and the egg reduction rate of 60.0% in the combined group were significantly higher than those of 8.1% for the SjC2 3DNA vaccine alone group and 37.9 %, And 3.8% worm reduction and 39.5% egg reduction (P <0.05) for the GST-HD 2 vaccine alone. Combination group antibody levels?