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为评估番茄灰霉病菌Botrytis cinerea对咯菌腈的抗性风险,就室内经紫外照射获得抗药突变体的方法及抗性突变体的生物学性状进行了研究。结果表明:番茄灰霉病菌分生孢子的紫外照射亚致死时间为90～120 s;经亚致死时间紫外照射后,4个亲本菌株中有2个菌株共产生了6个抗咯菌腈的突变体,其EC50值是亲本菌株的310倍以上,抗性突变频率为3.13×10-7;经紫外照射诱变获得的所有抗性突变体在菌丝生长速率、产孢量、产菌核能力及其在番茄果实上的致病性方面均比其亲本菌株明显降低。相关分析显示,所得抗咯菌腈突变体对氟啶胺、啶菌唑、啶酰菌胺和嘧霉胺无交互抗性。表明番茄灰霉病菌对咯菌腈的抗药性风险较低。
In order to assess the risk of resistance to fludioxonil in Botrytis cinerea, the methods of obtaining drug-resistant mutants by ultraviolet irradiation and the biological characters of resistant mutants were studied. The results showed that the sublethal death time of conidia of Botrytis cinerea was 90 ~ 120 s. After irradiation with sublethal time, 2 of the 4 parental strains produced 6 fluditonen mutations The EC50 value was 310 times higher than that of the parental strain, and the frequency of resistance mutation was 3.13 × 10-7. All of the resistant mutants obtained by UV irradiation had the highest mycelial growth rate, sporulation rate, And its pathogenicity in tomato fruit than its parent strain was significantly reduced. Correlation analysis showed that the obtained anticonazole mutants had no cross-resistance to fluazinam, dyclopyradine, boscalid and pyrimethanil. It showed that Botrytis cinerea had a lower risk of drug resistance to fludioxonil.