本文报道一种用高效液相色谱同时检测人血清及脑脊液中丙戊酸、扑痫酮、苯巴比妥、苯妥英钠、卡马西平5种抗癫痫药物的方法.反相C_(18)柱,乙腈/甲醇/磷酸盐缓冲液为流动相,检测波长210nm,样品用硫酸铵饱和的1mol/L盐酸及少量乙腈处理。范围内线性关系好,日内、日间变异小于5％.方法简便、快速、高灵敏度,适合于药代动力学研究及临床监测. This paper reports a method for the simultaneous determination of five antiepileptic drugs, valproic acid, epilepsy ketone, phenobarbital, phenytoin, and carbamazepine in human serum and cerebrospinal fluid (CSF) by high performance liquid chromatography , Acetonitrile / methanol / phosphate buffer as the mobile phase, detection wavelength 210nm, the sample was saturated with ammonium sulfate 1mol / L hydrochloric acid and a small amount of acetonitrile treatment. The linearity within the range is good, and the intra- and inter-day variation is less than 5%. The method is simple, rapid and sensitive and is suitable for pharmacokinetic studies and clinical monitoring.