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目的探讨银杏黄酮对颈淋巴阻断(CLB)后蛛网膜下腔出血(SAH)继发性脑损伤的保护作用。方法将Wistar大鼠随机分入对照组、SAH组、SAH+CLB组、SAH+CLB+生理盐水组、SAH+CLB+银杏黄酮50 mg组、SAH+CLB+银杏黄酮200 mg组,银杏黄酮经腹腔注射给予。制作CLB和SAH模型。于诱导SAH后3 d,用激光多普勒血流计测定局部脑血流量(rCBF),干湿重比较法测定脑组织含水率,免疫荧光法检测血红素氧合酶-1(HO-1)表达。结果 SAH后3 d rCBF显著降低,CLB可导致rCBF进一步下降,银杏黄酮可减轻rCBF下降的程度;SAH导致脑组织含水率升高,并在CLB时进一步增加,银杏黄酮对之具有拮抗作用;SAH促进脑组织HO-1表达,CLB后HO-1表达进一步增加,银杏黄酮促进CLB后SAH大鼠脑组织HO-1表达。结论 CLB可加重SAH后脑损伤,银杏黄酮对之具有一定缓解作用。
Objective To investigate the protective effect of ginkgetin on secondary brain injury following subarachnoid hemorrhage (SAH) in patients with cervical lymphatic block (CLB). Methods Wistar rats were randomly divided into control group, SAH group, SAH + CLB group, SAH + CLB + saline group, SAH + CLB + Ginkgetin 50 mg group, SAH + CLB + Ginkgetin 200 mg group, . Make CLB and SAH models. Local cerebral blood flow (rCBF) was measured by laser Doppler flowmetry and water content of brain tissue was measured by dry-wet weight comparison method at 3 days after induction of SAH. Heme oxygenase-1 (HO-1) )expression. Results rCBF decreased significantly at 3 d after SAH, and CLB decreased rCBF further. Ginkgetin could reduce the decrease of rCBF. SAH led to increase of water content of brain tissue and further increase of CLB. Ginkgetin could antagonize SAH Promote the expression of HO-1 in brain tissue, further increase the expression of HO-1 after CLB, and promote the expression of HO-1 in SAH rat brain after CLB. Conclusion CLB can aggravate brain injury after SAH, and Ginkgetin can relieve it.