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目的了解前列腺癌克隆演变(clonal evolution)过程中遗传学机制。方法采用激光显微切割技术从保存的石蜡包埋组织中获取基因组DNA;利用6个位于染色体8p12-21、8p22、17q21上的具有多态性的微卫星标记,对25例患者原发癌及相应转移灶中等位基因的缺失或保留进行分析。结果在24例可供信息的病例中,14例(58%)在原发癌及相应转移灶中所有位点均表现为相同的等位基因缺失或保留模式,而另外10例(42%)则显示不一致的等位基因缺失。这10例中有5例原发癌表现为等位基因保留而在相应转移灶则为缺失,另外5例在一个或一个以上的位点表现为原发癌等位基因缺失而在相应转移灶保留。结论前列腺癌在原发癌及相应转移灶遗传组成上的差异可能与其内在异质性、整体遗传不稳定性及克隆差异有关。
Objective To understand the genetic mechanism of clonal evolution in prostate cancer. Methods The genomic DNA was extracted from preserved paraffin-embedded tissue by laser microdissection. Six microsatellite markers with polymorphisms on chromosome 8p12-21, 8p22 and 17q21 were used to detect the expression of DNA in 25 patients with primary cancer and Deletion or retention of alleles in the corresponding metastases was analyzed. RESULTS Of the 24 available informative cases, 14 (58%) showed the same pattern of allelic loss or retention at all sites in primary cancer and corresponding metastases, while the other 10 (42% It shows an inconsistent allele deletion. In these 10 cases, 5 cases of primary cancer showed allele retention and deletion in the corresponding metastasis, while the other 5 cases showed deletion of the primary cancer allele at one or more sites and a corresponding metastasis Keep. Conclusion The differences in the genetic composition of primary carcinoma and its corresponding metastases may be related to its internal heterogeneity, overall genetic instability and clonal differences.