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目的:研究普萘洛尔对人血管内皮细胞的细胞毒作用、增殖影响及p-ERK表达影响,为进一步研究普萘洛尔治疗血管瘤机制提供基础参数。方法:以离体培养的人脐静脉内皮细胞(HUVEC-12)为模型,采用MTT法测定不同浓度的普萘洛尔对人血管内皮细胞的细胞毒作用、增殖影响及蛋白质免疫印迹法测定磷酸化细胞外信号调节激酶(ERK)变化。结果:普萘洛尔浓度为1-16ug/ml时对人血管内皮细胞有促进增殖作用,当普纳洛尔浓度为4ug/ml时最为明显,其增殖率为19.21%(P<0.001),未见细胞毒现象及p-ERK比表达无明显改变(P<0.05)。结论:细胞试验证实普萘洛尔浓度在1-16ug/ml的范围内,对正常人血管内皮细胞安全、无细胞毒作用。
OBJECTIVE: To study the effects of propranolol on the cytotoxicity, proliferation and p-ERK expression of human vascular endothelial cells, and provide the basic parameters for further study on the mechanism of propranolol in the treatment of hemangiomas. Methods: Human umbilical vein endothelial cells (HUVEC-12) cultured in vitro were used as a model to determine the effects of different concentrations of propranolol on the proliferation of human vascular endothelial cells by MTT assay and the effect of phosphorylation Changes in extracellular signal-regulated kinase (ERK). Results: Propranolol could promote the proliferation of human vascular endothelial cells when the concentration was 1-16 ug / ml, the most obvious was when the concentration of pranolol was 4 ug / ml, the proliferation rate was 19.21% (P <0.001) No cytotoxicity and p-ERK expression was not significantly changed (P <0.05). CONCLUSIONS: Cell tests have demonstrated that propranolol is present in a concentration range of 1-16 ug / ml and is safe and has no cytotoxic effect on normal human vascular endothelial cells.