建立鸡血清中恩诺沙星残留量的快速测定方法:荧光光度法。用甲醇沉淀蛋白对血清进行预处理,利用SDS-Mg2+-ENRX体系,鸡血清中恩诺沙星浓度在1.00~3.00μg·mL-1范围时,荧光强度与浓度呈线性关系,其回归方程为F=37.580 15+14.261 37C(μg·mL-1)(r=0.999 0),血清中恩诺沙星的平均回收率为97.76%,RSD=1.1%。所用方法简便、准确、快速,可用于鸡血清中恩诺沙星的测定。 Rapid determination of residual enrofloxacin in chicken serum by fluorescence spectrophotometry. Serum was pretreated by precipitation of protein with methanol. The linear relationship between fluorescence intensity and concentration was obtained when the concentration of enrofloxacin in chicken serum was in the range of 1.00-3.00 μg · mL-1 using SDS-Mg2 + -ENRX system. The regression equation was F = 37.580 15 + 14.261 37C (μg · mL-1) (r = 0.999 0). The mean recoveries of enrofloxacin in serum were 97.76% and RSD was 1.1%. The method used is simple, accurate and rapid and can be used for the determination of enrofloxacin in chicken serum.