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本试验采用周藤的尾孢菌产孢方法进行,供试尾孢菌共21种。方法是把人工培养的菌丝体或分生孢子接种到皮氏培养皿中马铃薯蔗糖琼脂表面,然后盖上聚二氯乙烯薄膜。在黑光灯连续性或周期性照射下恒温培养。试验结果,黑光灯的照射对十九种供试菌的孢子产生具诱导作用,其中一种有促进作用。有两种供试菌,无论在辐射还是黑暗条件下均不产生分生孢子。有些供试菌在连续性照射下产生的分生孢子比周期性照射的要多,而另外的供试菌,在两种照射处理中所产生的分生孢子数量相等。有些供试菌,人工培养所产生的分生孢子在形态特征上与寄主植物上所形成的多少有些差异。在供试的所有尾孢菌中,用这种方法培养出来的分生孢子都具很高的萌发力。因此这种方法被认为是培养尾孢菌分生孢子的最简单而有用的方法。
In this experiment, the method of sporulation of cercospora sp. Was carried out for 21 species of cercospores. The method consists of inoculating cultured mycelia or conidia onto the surface of potato sucrose agar in a petri dish and then covering with a polyvinylchloride film. In black light continuous or periodic exposure to culture under constant temperature. Test results, black light irradiation on the spores of nineteen kinds of test-induced production, one of which can promote the role. There are two kinds of test bacteria, no conidia are produced in the radiation or dark conditions. Some of the test strains produced more conidia than the ones irradiated periodically, whereas the other tested bacteria produced equal numbers of conidia in both irradiation treatments. Some test bacteria, artificial culture conidia generated by the morphological characteristics of the host plant with the formation of somewhat different. Conidia cultured in this way all have high germination power among all cercospores tested. Therefore, this method is considered to be the simplest and most useful method for culturing cercospores conidia.