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目的为了加强麻疹病毒基因型的监测,2013年北京市开展了麻疹病毒的核酸检测。方法采用荧光PCR方法进行病毒核酸鉴定,然后对于核酸阳性标本进行RT-PCR(Reverse Transcription PCR,RT-PCR)扩增,扩增产物进行测序及序列比对分析。结果 2013年北京市西城区共采集麻疹疑似病例为41例,获得RT-PCR产物21例,经过序列分析比对,有16例为H1基因型。本研究获得的16例H1基因型的麻疹病毒基因片段序列与世界卫生组织H基因型代表株MVi/Hunan.CHN/0.93/7/H1,在基因亲缘性关系树上同属一个分支,核苷酸同源性为97.1%~97.6%,氨基酸同源性为95.3%~98.0%。结论 2013年北京市麻疹病例明显增多,以本土基因型病例为主,进一步加强麻疹病例控制和预防,对于实现消除麻疹的计划具有重要意义。
Objective In order to strengthen the monitoring of the genotype of measles virus, Beijing carried out the detection of measles virus nucleic acid in 2013. Methods Fluorescent PCR was used to identify the virus nucleic acid. Then RT-PCR (Reverse Transcription PCR) was used to amplify the positive samples. The amplified products were sequenced and compared by sequence analysis. Results A total of 41 suspected measles cases were collected in Xicheng District of Beijing in 2013. Twenty-one cases of RT-PCR products were obtained. Among the 16 cases, H1 was genotyped by sequence analysis. The 16 Hl genotypes of the measles virus obtained in this study were identical to the WHO H genotype MVi / Hunan.CHN / 0.93 / 7 / H1 in the gene relationship tree, and the nucleotide The homology was 97.1% ~ 97.6% and the amino acid homology was 95.3% ~ 98.0%. Conclusion The measles cases in Beijing increased significantly in 2013. The cases of local genotypes were the major ones in Beijing. The control and prevention of measles cases was further strengthened, which is of great significance for the plan to eliminate measles.