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为探明桃小食心虫取食危害对不同品种苹果果实蛋白质含量和防御酶活性的影响,田间条件下采用人工接虫和针刺模拟危害苹果果实,处理12 d后调查果实受害程度并测定蛋白质含量及超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性,分析了果实蛀孔数与可溶性蛋白质和防御性酶活性的关系。结果表明:人工接虫后,“嘎啦”的蛀孔数显著多于“乔纳金”和“金帅”。桃小食心虫危害后苹果果实蛋白质含量变化不大,均显著低于针刺模拟处理;SOD活性显著高于针刺模拟处理(P<0.05);POD活性都显著增加,“乔纳金”、“金帅”的变化显著大于“嘎啦”;CAT活性都与对照相比无显著变化,均高于针刺模拟处理,CAT酶活性与苹果蛀孔数呈显著正相关。桃小食心虫危害可以诱导寄主产生一系列反应,蛋白质含量和应激防御酶活性均发生了变化,其中,防御性酶可能在苹果防御桃小食心虫危害中发挥着重要作用。
In order to find out the effects of feeding on the fruit protein content and defense enzyme activities of different varieties of apple pests, the artificial fruit pests and acupuncture were used to simulate the damage to the apple fruits under field conditions. After 12 days of treatment, the degree of fruit damage was investigated and the protein content And the activity of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), and analyzed the relationship between the number of fruit holes and soluble protein and defensive enzyme activity. The results showed that there were significantly more worm holes than those of “Jonathan” and “Jin Shuai” after artificial insects. The contents of protein in fruit of apple peach did not change much, which were significantly lower than that of acupuncture simulation. SOD activity was significantly higher than that of acupuncture simulation (P <0.05); POD activity increased significantly, The change of CAT activity was significantly greater than that of GA. CAT activity had no significant change compared with the control, which was higher than that of acupuncture simulation treatment. The CAT activity had a significant positive correlation with the number of apple hole infestation. Peach beetle damage can induce a host of host reactions, with changes in both protein content and stress-defending enzyme activity, of which defensive enzymes may play an important role in the defense of apple against the pest.