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目的 了解MDR 1基因表达与肺癌细胞耐药性的关系。方法 用FUGENTM6转染试剂将MDR 1基因导入小鼠Lewis肺腺癌细胞株 ( 3LL) ,建立了耐药细胞株 ( 3LL MDR 1)。用MTT方法测定细胞耐药性并观察异搏定对耐药的逆转效应 ,MDR 1基因表达产物P gp采用免疫组化方法观察 ,应用流式细胞仪分析其对示踪剂罗丹明1 2 3的摄取与排泄。结果 建立了一株能稳定表达P gp和对长春生物碱类、鬼臼毒素类具有显著耐药性的细胞株。免疫组化证实耐药细胞高度表达P gp糖蛋白。异搏定能部分逆转该细胞株的耐药性 ,且效应随着剂量递增 ,流式细胞仪测定显示耐药细胞对示踪剂的排泄作用显著高于亲代细胞。结论 通过基因转染实验 ,证明MDR 1基因表达与肺癌细胞多药耐药有关
Objective To investigate the relationship between MDR 1 gene expression and drug resistance in lung cancer cells. Methods MDR 1 gene was transfected into mouse Lewis lung adenocarcinoma cell line (3LL) with FUGENTM6 transfection reagent to establish a drug resistant cell line (3LL MDR 1). MTT assay was used to measure the drug resistance of cells and the reversal effect of verapamil on drug resistance was observed. The expression of MDR 1 gene P gp was observed by immunohistochemical method. Flow cytometry was used to analyze the effect of trastuzumab on rhodamine 1 2 3 Ingestion and excretion. Results A cell strain that could stably express P gp and had significant resistance to vinca alkaloids and podophyllotoxins was established. Immunohistochemistry confirmed that resistant cells highly express Pgp glycoprotein. Verapamil partly reversed the drug resistance of this cell line, and the effect increased with dose. Flow cytometry showed that the excretion of drug-resistant cells to tracer was significantly higher than that of the parental cells. Conclusion Through gene transfection experiments, MDR 1 gene expression and lung cancer multidrug resistance