经分离纯化获得纯菌株并在培养基上产生了分生孢子．分别用病死枝上子实体内挑取的子囊孢子、纯培养产生的分生孢子制备孢子悬浮液，在无病区烫伤接种健康华山松枝条和表面消毒的离体枝条，均使之发病并出现了与自然发病相同的病状、病症（分生孢子器和分生孢子）．经显微镜检视计测，鉴定出松黑腐皮壳（ＶａｌｓａｐｉｎｉＦｒ．）和其无性型松腐皮壳囊孢（ＣｙｔｏｓｐｏｒａｐｉｎｉＤｅｓｍ．）． Pure strains were isolated and purified and conidia were produced on the medium. The ascospores were picked from the fruiting bodies of diseased branches and the conidia produced by the pure cultures were used to prepare spore suspensions. The diseased area was scalded and inoculated with healthy Chinese pine branches and surface disinfected detached shoots, which caused the disease and appeared The same with the natural onset of the disease, disorders (conidia and conidia). Microscopic examination revealed that Valsapini Fr. and its anamorph Cytospora pini Desm. Were identified.