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目的制备单甲氧基聚乙二醇-聚乳酸-羟基乙酸纳米粒(monomethoxy polyethylene glycol-poly-actic coglycolic acid-nanoparticles,mPEG-PLGA-NPs)并研究其理化性质及体外抗黏性能,考察人宫颈癌细胞HeLa对mPEG-PLGA-NPs的摄取能力。方法采用溶剂扩散法制备mPEG-PLGA-NPs,测定其平均粒径和zeta电位。采用黏蛋白结合法,考察mPEG-PLGA-NPs的体外抗黏性能。以香豆素-6(coumarin 6)为荧光标记物,通过共聚焦显微镜及HPLC法进行HeLa细胞的体外摄取研究。结果 mPEG-PLGA-NPs的平均粒径为(106.2±4.3)nm,Zeta电位为-(12.40±0.11)mV。PLGA-NPs的抗黏蛋白黏附率为35.5%,而mPEG-PLGA-NPs的抗黏蛋白黏附率为92.5%,比PLGA-NPs高3倍左右。相同孵育时间内,HeLa细胞对mPEG-PLGA-NPs的摄取量是PLGA-NPs的近2倍,HeLa细胞对PLGA-NPs和mPEG-PLGA-NPs的摄取量与孵育时间成明显的依赖关系。结论 mPEG-PLGA-NPs具有较强的抗黏性;HeLa细胞对mPEG-PLGA-NPs的摄取明显高于PLGA-NPs(P<0.01),表现出更好的细胞亲和性。
Objective To prepare monomethoxy polyethylene glycol-poly-actic coglycolic acid-nanoparticles (mPEG-PLGA-NPs) and study its physical and chemical properties and in vitro anti-stick properties. Uptake capacity of cervical cancer cell HeLa to mPEG-PLGA-NPs. Methods mPEG-PLGA-NPs were prepared by solvent diffusion method. The average particle size and zeta potential were measured. Mucin binding assay was used to investigate the anti-adhesion properties of mPEG-PLGA-NPs in vitro. In vitro studies of HeLa cells by confocal microscopy and HPLC were performed using coumarin 6 as a fluorescent marker. Results The average particle size of mPEG-PLGA-NPs was (106.2 ± 4.3) nm and the Zeta potential was - (12.40 ± 0.11) mV. The anti-mucin adhesion rate of PLGA-NPs was 35.5%, while that of mPEG-PLGA-NPs was 92.5%, about 3 times higher than that of PLGA-NPs. In the same incubation time, uptake of mPEG-PLGA-NPs by HeLa cells was nearly double that of PLGA-NPs. The uptake of PLGA-NPs and mPEG-PLGA-NPs in HeLa cells was significantly dependent on the incubation time. CONCLUSION: mPEG-PLGA-NPs have strong anti-adhesion properties. The uptake of mPEG-PLGA-NPs by HeLa cells is significantly higher than that of PLGA-NPs (P <0.01), showing better cell affinity.