The present study was designed to investigate the antithrombotic effects and underlying mechanisms of the effective components group(ECG) of Xiaoshuantongluo recipe(XECG) and to further verify the rationality and feasibility of ECG-guided methodology in traditional Chinese medicine(TCM) research. The arterial thrombosis model induced by ferric chloride(FeC l3) oxidation and the venous thrombosis model induced by inferior vena cava ligation were established to evaluate the antithrombotic potential of XECG. Our results indicated that XECG significantly prolonged the time to occlusion, activated partial thromboplastin time(APTT), and prothrombin time(PT), and markedly inhibited adenosine diphosphate(ADP)-induced platelet aggregation in the 20% Fe Cl3-induced arterial thrombosis model. The superoxide dismutase(SOD) activity was significantly increased and the levels of malondialdehyde(MDA) and nitric oxide(NO) were dramatically decreased in the plasma of arterial thrombosis rats after XECG treatment for 12 days. Furthermore, XECG markedly reduced the weight of thrombus formed by inferior vena cava ligation. Additionally, XECG exhibited 2,2-diphenyl-1-picrylhydrazyl(DPPH) free radical scavenging activity and protective effect on mitochondrial lipid peroxidation. In summary, XECG played an important role in the prevention of thrombosis through interacting with multiple targets, including inhibition of platelet aggregation and coagulation and repression of oxidative stress. The ECG-guided methodology was validated as a feasible tool in TCM research. The present study was designed to investigate the antithrombotic effects and underlying mechanisms of the effective components group (ECG) of Xiaoshuantongluo recipe (XECG) and to further verify the rationality and feasibility of ECG-guided methodology in traditional Chinese medicine (TCM) research. The arterial thrombosis model induced by ferric chloride (FeCl3) oxidation and the venous thrombosis model induced by inferior vena cava ligation were established to evaluate the antithrombotic potential of XECG. Our results indicated that XECG significantly prolonged the time to occlusion, activated partial thromboplastin time ( APTT), and prothrombin time (PT), and markedly inhibited adenosine diphosphate (ADP) -induced platelet aggregation in the 20% Fe Cl3-induced arterial thrombosis model. The superoxide dismutase (SOD) activity was significantly increased and the levels of malondialdehyde MDA) and nitric oxide (NO) were dramatically decreased in the plasma of arterial thrombosis rats after XECG XDPG free radical scavenging activity and protective effect on mitochondrial lipid peroxidation. In, XECG markedly reduced the weight of thrombus formed by inferior vena cava ligation. In, XECG exhibited 2, 2-diphenyl-1-picrylhydrazyl summary, XECG played an important role in the prevention of thrombosis through interacting with multiple targets, including inhibition of platelet aggregation and coagulation and repression of oxidative stress. The ECG-based method was validated as a feasible tool in TCM research.