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目的观察鬼针草总黄酮(TFB)对大鼠实验性脑出血后脑组织损伤的影响,探讨其对大鼠脑损伤的保护机制。方法采用大脑立体定位技术,取不抗凝血液50μL缓慢注入尾状核,建立大鼠实验性脑出血模型,观察TFB对脑组织含水量、全血粘度及脑匀浆中超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)等生化指标的影响。结果 TFB高、中、低剂量组均能显著改善脑出血大鼠脑含水量、全血粘度,高、中剂量组能明显升高脑组织SOD活性,降低MDA、NO含量(P<0.05)。结论 TFB对实验性脑出血大鼠脑损伤的保护作用可能与其减轻脑水肿、改善血肿周围组织的微循环状态、减轻细胞膜脂质过氧化损伤、抑制NO的产生有关。
Objective To observe the effect of total biloba flavonoids (TFB) on brain injury after experimental intracerebral hemorrhage (ICH) in rats and its protective mechanism on brain injury in rats. Methods The cerebrum stereotaxic technique was used to inject the caudate nucleus into the caudate nucleus without anticoagulant. The experimental cerebral hemorrhage model was established in rats. The content of water in the brain tissue, whole blood viscosity and the activity of superoxide dismutase (SOD) ), Malondialdehyde (MDA), nitric oxide (NO) and other biochemical indicators. Results High, medium and low doses of TFB could significantly improve the brain water content of rats with cerebral hemorrhage. The whole blood viscosity, high and middle dose groups could significantly increase the activity of SOD and decrease the content of MDA and NO in brain tissue (P <0.05). Conclusions The protective effect of TFB on brain injury induced by intracerebral hemorrhage in rats may be related to the reduction of brain edema, the improvement of the microcirculation of the perihematoma tissue, the reduction of lipid peroxidation injury and the production of NO.