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9例慢性痛疒人,腰穿收集两次脑脊液,一次是在电刺激前12～18小时,疒人感痛而未采取镇痛措施时;另一次是在用表面电极刺激穴位45分钟后的30分钟以内收集,每次收集10ml,经离心、超过滤,再用分子筛Sephadex G-10柱层析,得组分Ⅰ和Ⅱ,冰冻干燥后用受体结合法测定其中内啡肽。用甲硫氨酸—脑啡肽经同法柱层析,作出标准曲线,脑脊液中内啡肽含量用甲硫氨酸—脑啡肽的pmoles/ml表示。根据作者1976年的工作:人脑脊液中,内啡肽的组分Ⅰ和Ⅱ占总内啡肽的75％以上;在无慢性痛的疒人,组分
Nine patients with chronic pain were collected twice in the lumbar spine. One time, 12-18 hours before the electrical stimulation, they did not take any pain-relieving measures. The other time was 45 minutes after stimulating the acupoint with the surface electrode Collect 30 ml each time, and collect 10 ml each time. Centrifuge and filter through ultrafiltration, and then use molecular sieve Sephadex G-10 column chromatography to obtain fractions I and II. After freeze-drying, endorphins were determined by receptor binding assay. Methionine - enkephalin by the same method of column chromatography to make a standard curve, cerebrospinal fluid endorphin content with methionine - enkephalin pmoles / ml said. According to the author’s 1976 work: human cerebrospinal fluid, the endorphin components I and II accounted for more than 75% of the total endorphin;