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目的测定眼镜蛇毒细胞毒素（ＣＶ－ＣＴＸ）对人癌细胞株的细胞抑制作用，以期能简便准确地反映其抗癌活性。方法应用溴化二苯四偶氮盐（ＭＴＴ）法测定在ＣＶ－ＣＴＸ作用下体外培养的人胃癌细胞株（ＳＧＣ－７９０１）和人肝癌细胞株（ＳＭＣ）的存活情况和其量效关系。结果（１）ＳＭＣ、ＳＧＣ－７９０１的活细胞数与其分解ＭＴＴ的量成正线性关系。（２）ＣＶ－ＣＴＸ对ＳＭＣ、ＳＧＣ－７９０１癌细胞株的细胞抑制作用呈良好的量效关系，ＩＣ５０分别为２．４５和１．２４μｇ／ｍｌ。结论（１）用ＭＴＴ法测定癌细胞的增殖和衰减是可靠的。（２）应用ＭＴＴ法证实ＣＶ－ＣＴＸ对体外培养的人癌细胞的杀伤程度可作为其抗癌活性指标。
Objective To determine the cytotoxicity of cobra venom cytotoxin (CV-CTX) on human cancer cell lines in order to reflect its anticancer activity easily and accurately. Methods The survival of human gastric cancer cell line SGC-7901 and human hepatocellular carcinoma cell line (SMC) cultured under the action of CV-CTX and the dose-response relationship were determined by MTT assay. Results (1) The number of viable cells in SMC and SGC-7901 had a positive linear relationship with the amount of MTT. (2) CV-CTX showed a good dose-response relationship with SMC and SGC-7901 cancer cell lines with IC50 of 2.45 and 1.24 μg / ml, respectively. Conclusion (1) MTT assay of cancer cell proliferation and attenuation is reliable. (2) MTT assay confirmed that CV-CTX can kill human cancer cells cultured in vitro as an indicator of anticancer activity.