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本文旨在探讨细胞角蛋白和波蛋白在大鼠发育中睾丸内的表达,特别是在支持细胞内的表达.阴栓龄17～20天的Wistar胎鼠和生后1、5、10、15、20和40天的幼鼠购自中国医学科学院实验动物中心,生后1天的仔鼠及胎鼠取右侧睾丸于4%多聚甲醛中浸泡固定.生后5天以上的幼鼠先经心脏灌流固定,再取睾丸浸泡固定.石蜡包埋后组织块作5μm厚切片.PAP法或ABC法显示细胞角蛋白,Biotin-Streptoavidin法显示波蛋白.兔抗牛角蛋白多克隆抗体和小鼠抗猪波蛋白单克隆抗体均为DAKO公司产品.结果如下:(1)17天龄胎鼠睾丸可见两种中间纵蛋白的表达.角蛋白主要见于表面上皮,纵隔及其附近的睾丸索(testicular cords)内的上皮细胞以及其它睾丸索的支持细胞.在纵隔以外的睾丸索,角蛋白的反应弱,只见于支持细胞的核下区.波蛋白分布于白膜、问质及血管内皮.在睾丸索内则分布在支持细胞的核下区.(2)随年龄增长,睾丸索内支持细胞的角蛋白表达减弱.纵隔及其附近的睾丸索除外,它们发展成为直细精管和睾丸网.生后15天以后,睾丸索(曲细精管)支持细胞的角蛋白用PAP法已不能显示.(3)用ABC法,在出生后15天以上的曲细精管内确可见到少数角蛋白阳性细胞,大多数支持细胞的波蛋白表达增强.本结果提示,支持细胞可能有两种类型.随着发育,大多数支持细?
This article aims to investigate the expression of cytokeratin and wave protein in developing rat testis, especially in the supporting cells.Wistar fetus aged 17 to 20 days and postnatal day 1,5,10,15 , 20- and 40-day-old pups were purchased from the Laboratory Animal Center of Chinese Academy of Medical Sciences, and the right testes of the pups and fetuses of 1 day after birth were soaked in 4% paraformaldehyde and fixed for more than 5 days Fixed by heart perfusion, and then fixed in testis soaked in paraffin-embedded tissue section for 5μm thick sections.PAP method or ABC method showed cytokeratin, Biotin-Streptoavidin method showed wave protein.Human anti-keratin polyclonal antibody and mouse The anti-porcine monoclonal antibodies were all DAKO products.The results are as follows: (1) The expression of two intermediate longitudinal proteins was observed in 17-day-old fetus testes.The keratin was mainly found on the surface epithelium, mediastinal and nearby testicular cords) and other testicular cord supporting cells in the mediastinal testis cord, keratin reaction is weak, only found in the supporting cells of the subgardal area. Wave protein distribution in the white film, ask the quality and the endothelium. Testicular cord is located in the supporting cells under the nucleus . (2) With age, the keratin expression in supporting cells of the testicular cord weakened. In addition to the testicular cord in and around the mediastinum, they developed into seminiferous tubules and testicular neoplasms. After 15 days, the testicular cord (3) With ABC method, a small number of keratin-positive cells were found in the seminiferous tubules more than 15 days after birth, most of which support the wave protein expression of the cells The results suggest that there are likely to be two types of supporting cells.With development, most supports fine?