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[目的]观察扶正泄浊饮对腺嘌呤致大鼠慢性肾衰竭肾功能影响。[方法]使用随机平行对照方法,将50只健康雄性wistar大鼠按随机数字表随机分为5组(10只/组),模型组、扶正泄浊饮组、析清胶囊组2.5%腺嘌呤悬溶液(200mg/Kg.d),正常组等体积纯净水,连续灌胃5周。第6周开始,各组按10mL/Kg.d容量药物灌胃干预,正常组和模型组等体积纯净水,连续灌胃10周。药物干预10周后,腹主动脉取血,测定血常规、肾功,用ELISH法检测大鼠TGF-β1和MCP-1。10周后处死,即刻取肾脏组织,甲醛溶液固定,切片,HE染色,封固,光镜观察。观测状态、重量、饮食、二便等。[结果]扶正泄浊饮具有降低肌酐、尿素氮,同时能改善贫血,对比析清胶囊在改善肾功方面有一定的优势(P<0.05)。[结论]扶正泄浊饮能有效干预造模大鼠CRF,改善一般状态,延缓CRF发展,降低肌酐、尿素氮,改善贫血,与析清胶囊比较在改善肾功方面有一定优势;其机制可能为抑制TGF-β1表达和MCP-1的沉积,进而减缓CRF的进一步发展。
[Objective] To observe the influence of Fuzheng Xiezhuo Yin on renal function of chronic renal failure induced by adenine in rats. [Methods] Fifty healthy male wistar rats were randomly divided into five groups (n = 10) according to the randomized parallel control group. The model group, Fuzheng Xiezhuo Decoction group and Qing Qing Capsule group were given 2.5% adenine Suspension solution (200mg / Kg.d), the normal group of equal volume of pure water, continuous gavage for 5 weeks. At the beginning of the sixth week, each group was given gavage by 10 mL / Kg.d, and the normal group and the model group were given the same volume of pure water for 10 weeks. After 10 weeks of drug intervention, blood was taken from abdominal aorta, blood routine and renal function were measured. TGF-β1 and MCP-1 were detected by ELISH in 10 weeks and sacrificed immediately. Kidney tissues were immediately fixed and formaldehyde solution was fixed. Dyeing, sealing, light microscopy. Observed state, weight, diet, secondhand and so on. [Result] Fuzheng Xiezhuo Decoction could reduce creatinine and urea nitrogen and improve anemia at the same time. Comparing with those of Qingzheng Capsule in improving renal function (P <0.05). [Conclusion] Fuzheng Xiezhuo Decoction can effectively intervene the CRF of model rats, improve the general condition, delay the development of CRF, lower creatinine and urea nitrogen, and improve anemia, which may have some advantages in improving renal function compared with those of XQ capsule. The mechanism may be To inhibit TGF-β1 expression and MCP-1 deposition, thereby slowing the further development of CRF.