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应用抗HBV-X基因区编码蛋白的单克隆抗体,经免疫印迹技术和免疫组化法对30侧慢性乙肝患者肝内 HBxAg 存在状况的研究。结果表明 HBxAg 阳性率高低与肝组织的慢性化程度一致:CPH 为14.3%,CAH26.7%,CAH+LC50%。在血清抗-HBe 阳性者中阳性率为50%,高于 HBeAg阳性者(21.4%)或 HBsAg 伴抗-HBc 阳性者(25%)。本文提示抗-HBx 的存在可能与 HBVDNA的整合有关。
The anti-HBV-X gene region encoding protein monoclonal antibody was used to detect the presence of HBxAg in the liver of 30 patients with chronic hepatitis B by Western blotting and immunohistochemistry. The results showed that the positive rate of HBxAg consistent with the degree of chronic liver tissue: CPH was 14.3%, 26.7% CAH, CAH LC50%. The positive rate was 50% in serum anti-HBe positive patients, higher than those with HBeAg positive (21.4%) or HBsAg with anti-HBc positive (25%). This article suggests that the presence of anti-HBx may be related to the integration of HBVDNA.