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为了研究化学诱变技术在棉花种质资源创制中的效果及探索适宜的新疆早熟陆地棉种子诱变体系,本研究利用不同浓度、不同时间组合甲基磺酸乙酯(EMS)溶液处理新疆早熟陆地棉材料223种子。结果显示:在EMS浓度为1.0%,处理2 h是最有效的处理组合;在M1代中出现了叶片形状差异、第一果节真叶数量差异和植株矮化、双头等变异性状;M2代中获得双茎、子叶黄斑、无顶端等变异性状植株。对部分M2代及变异植株进行SSR分析表明,变异植株与对照在电泳条带上存在差异,一定程度上证实了在DNA水平上发生了变异,说明了变异的真实性。本研究获得的适宜诱变体系可为深入开展棉花种质创新及相应的机理研究提供研究基础。
In order to study the effect of chemical mutagenesis in the creation of cotton germplasm resources and to explore the suitable seed germplasm system of Xinjiang precocious G. hirsutum, we studied the effects of different concentrations of ethyl methyl sulfonate (EMS) solution on the precociousness of Xinjiang Gossypium hirsutum material 223 seeds. The results showed that the most effective treatment combinations were 1.0% EMS and 2 h EMS. In the M1 generation, leaf shape difference, number of true leaves in the first fruit section and plant dwarf and double-head variation were observed. In the M2 generation In the double stems, cotyledons macular, topless and other variability traits plants. SSR analysis of some M2 generation and mutant plants showed that there was a difference in the electrophoresis bands between the mutant plants and the control plants, which confirmed to some extent that there was a mutation at the DNA level, indicating the authenticity of the mutation. The appropriate mutagenesis system obtained in this study can provide the basis for further research on cotton germplasm innovation and its corresponding mechanism.