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自江苏省主要产棉区分离到棉花黄萎病菌(V.dahliae)17个菌株,在棉花苗期,用一定浓度的孢子悬浮液蘸根接种,接种后在23—25℃的温室内培养,观察发病的反应型.根据各菌株在海岛棉米努菲416、中棉江阴白籽、陆地棉陕西721、鲁棉1号及徐州142等不同抗性的棉花品种上的反应型,测定菌株致病力的强弱显然不同,可分为致病力强的Ⅰ型、致病力弱的Ⅲ型和致病力中等的Ⅱ型.致病力强的Ⅰ型引起落叶,亦称作落叶型菌株,目前只在我国局部地区发现。除棉花外,致病力强弱不同的菌株接种在大豆、豇豆等11种植物上的症状反应也有明显的盖异.
Seventeen strains of Verticillium dahliae were isolated from the main cotton producing areas of Jiangsu Province. In the cotton seedling stage, spores were inoculated with a certain concentration of spore suspension and inoculated in the greenhouse at 23-25 ℃ after inoculation. According to the reaction patterns of each strain on different cotton cultivars with different resistances, such as island cotton Minutiae 416, Zhongmianjiang Yin white seed, Upland cotton Shaanxi 721, Lumian 1 and Xuzhou 142, The strength of the disease is obviously different, can be divided into strong pathogenic type Ⅰ, the pathogenicity of weak type Ⅲ and moderate pathogenic type Ⅱ. Severe pathogenic type Ⅰ caused by deciduous, also known as deciduous Strains, currently only found in some areas of our country. In addition to cotton, pathogenicity of different strains inoculated in soybean, cowpea and other 11 kinds of plants on the symptom response also obvious cover different.