目的　通过术前检测细胞DNA倍体情况 ,探讨DNA指数 (DI)在胰腺癌诊断中的应用价值。方法　选取疑诊胰腺癌病人 30例行术前B超引导经皮穿刺 ,涂片行HE和改良Feulgen法染色 ,用ICM进行DNA含量测定。结果　在最终确诊的 2 7例胰腺癌中 ,异倍体 2 5例 ,其中HE诊断的敏感性是 6 6 6 7% ,DI值诊断的敏感性为92 6 % ,其差异具有统计学意义 (P <0 0 5 ) ,良性病变 3例均不属异倍体。在HE漏诊的 9个病例中 ,8例根据DI值可作出正确判断 ,3例良性的DI值无假阳性结果。结论　细针穿刺细胞DNA含量检测在术前胰腺癌的确诊中有着较重要的应用价值 Objective To evaluate the value of DNA index (DI) in the diagnosis of pancreatic cancer by detecting the DNA ploidy before surgery. Methods Thirty patients with suspected pancreatic cancer underwent preoperative B-guided percutaneous puncture. The smears were stained with HE and modified Feulgen method, and the content of DNA was determined by ICM. Results Of the 27 final diagnosed cases of pancreatic cancer, aneuploidy had 25 cases, of which the diagnostic sensitivity of HE was 66.67% and the diagnostic sensitivity of DI was 92.6%, the difference was statistically significant ( P <0 05), benign lesions in 3 cases are not aneuploid. Of 9 cases missed by HE, 8 cases were able to make correct judgments based on DI values ​​and 3 cases of benign DI values ​​did not have false positive results. Conclusion The DNA content of fine needle aspiration cells has important value in the diagnosis of preoperative pancreatic cancer