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AIM:To investigate FasL expression in hilarcholangiocarcinoma tissues and culturedcholangiocarcinoma cells,and to assess its ability to induceapoptosis.METHODS:We studied the expression of FasL by humanhilar cholangiocaroinomas tissues byimmunohistochemistry,and the QBC939 cholangiocarcinomacell line by FIT-PCR,immunohistochemistry,and WesternBlot.TUNEL and flow cytometry were used to detectapoptotic cells.RESULTS:Prevalent expression of FasL was detected in 39resected hilar cholangiocarcinoma tissues.TUNEL slainingdisclosed a high level of call death among lymphocytesinfiltrating FasL positive areas of tumor.FasL mRNA andprotein expressions in cholangiocarcinoma cells couldinduce Jurkat cells.OONCLUSION:Hilar cholangiocarcinomas may eludeimmunological surveiliance by inducing,via Fas/FasLsystem,the apoptosis of activated lymphocytes.
AIM: To investigate FasL expression in hilarcholangiocarcinoma tissues and culturedcholangiocarcinoma cells, and to assess their ability to induceapoptosis.METHODS:We studied the expression of FasL by humanhilar cholangiocaroinomas tissues byimmunohistochemistry, and the QBC939 cholangiocarcinomacell line by FIT-PCR, immunohistochemistry, and WesternBlot. TUNEL and flow cytometry were used to detectapoptotic cells.RESULTS:Prevalent expression of FasL was detected in 39resected hilar cholangiocarcinoma tissues.TUNEL slainingdisclosed a high level of call death among lymphocytesinfiltatingFasL positive areas of tumor.FasL mRNA and protein expressions in cholangiocarcinoma cells couldinduce Jurkat cells .OONCLUSION: Hilar cholangiocarcinomas may eludeimmunological surveiliance by inducing,via Fas/FasLsystem,the apoptosis of activated lymphocytes.