由于对SLE患者的单核细胞(MNC)产生IL-2的功能及对IL-2的应答性是否正常有争论。作者检查了68名SLE患者及20名正常人的MNC在不同条件下产生IL-2的能力及其对IL-2的应答性。取1:2稀释的EDTA血,密度梯度离心后分离MNC,加入ConA或加入ConA和乙酸肉豆蔻佛波醇(phorbol-myristateacetate,PMA),分别在利于细胞增殖但限制IL-2产生的含血清的培养液(亚适宜条件,Suboptimal condition)中及不利于 There is a debate about whether IL-2 production by monocytes (MNCs) in SLE patients and the response to IL-2 are normal. The authors examined the ability of 68 SLE patients and 20 normal human MNCs to produce IL-2 under different conditions and their response to IL-2. Take 1: 2 diluted EDTA blood, centrifuge density gradient centrifugation MNC, add ConA or add ConA and phorbol-myristate acetate acetate (PMA), were conducive to cell proliferation but limit the IL-2-producing serum (Suboptimal conditions) and not conducive to