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目的:以小肠黏膜下层(SIS)吸附磁颗粒作为热疗介质,研究靶向磁热疗对小鼠结肠癌皮下移植模型的作用。方法:选择Balb/c雌性小鼠66只,4～6周龄,体质量22～25g,CT26结肠癌细胞皮下注射建立小鼠模型。将磁流体颗粒沉淀于置备好的SIS上,手术植入实验组小鼠结肠癌组织周围进行包裹。每24h将实验组小鼠暴露在突变磁场下60min,共3次。对应设置空白对照组,SIS对照组,磁流体对照组,磁场对照组进行对比研究,每组11只。观察各组小鼠的肿瘤生长情况和小鼠生存时间,检测各组小鼠各主要脏器的病理变化。结果:磁热疗作用后,实验组肿瘤中心温度在15min后稳定在43℃,而正常组织温度则始终稳定在24℃。病理检测显示,各组小鼠各主要脏器未见明显结构异常。与各对照组相比较,实验组小鼠肿瘤生长受到显著抑制,P<0.05;生存时间显著延长,P<0.05。结论:以SIS吸附磁颗粒作为热疗介质的靶向磁热疗能显著抑制小鼠结肠癌模型的肿瘤生长,延长小鼠生存时间。靶向磁热疗的进一步研究有可为结肠癌治疗提供新的方法。
Objective: To study the effect of targeted magnetic hyperthermia on murine colon cancer subcutaneous transplantation using small particles of intestinal submucosa (SIS) magnetic particles as hyperthermia medium. Methods: Sixty Balb / c female mice were selected, 4 to 6 weeks old, body weight 22 ~ 25g, and CT26 colon cancer cells were injected subcutaneously to establish a mouse model. The magnetic fluid particles were precipitated on the prepared SIS, and the experimental group was implanted around the colon cancer tissues of mice. The experimental mice were exposed to a magnetic field for 60 min every 24h for 3 times. Corresponding to set the blank control group, SIS control group, magnetic fluid control group, magnetic field control group were compared, 11 in each group. The growth of mice in each group and the survival time of mice were observed, and the pathological changes of the major organs in each group were detected. Results: After the effect of magnetic hyperthermia, the tumor center temperature in the experimental group stabilized at 43 ℃ after 15min, while the normal tissue temperature was stable at 24 ℃. Pathological examination showed that no significant structural abnormalities were found in all major organs of mice in each group. Compared with the control group, the experimental group mice tumor growth was significantly inhibited, P <0.05; survival time was significantly longer, P <0.05. Conclusion: The targeted magnetic hyperthermia with SIS adsorbed magnetic particles as a hyperthermia medium can significantly inhibit the tumor growth and prolong the survival of mice with colon cancer. Further studies targeting magnetothermal therapy may provide new ways of treating colon cancer.